Fig.-The staining of human PBMC induced by CD3 and CD28 antibodies shows the results of Mouse IgG1 Isotype control, PE-Cy7 and Anti-Human CD4, APC on the left, and the results of anti-human CD223, PE-Cy7 (clone: oti5d8) and Anti-Human CD4, APC on the right. All cells are gated.

Overview of LAG3 molecular target information

Molecular name: LAG3, lymphocyte activating 3.

Gene family: CD molecules; Immunoglobulin like domain containing

Alias: CD223

Full name: lymphocyte-activation gene 3

Review of LAG3 molecular targets

CD223, the lymphocyte activation gene 3(LAG-3), has higher affinity for MHC-II than CD4, so it is considered to be related to the negative regulation of T cell activation and steady-state proliferation. LAG-3 has been reported to be expressed on activated T cells (including regulatory T cells) and NK cells. LAG-3 is usually expressed more on CD8+T cells than CD4+T cells. The co-expression of LAG-3 and CD49b is recommended to identify human and mouse Tr1 cells.

Fig.-staining of human PBMC induced by antibodies to CD3 and CD28. On the left, the results of Mouse IgG1 Isotype control, FITC and Anti-Human CD4, APC are shown, while on the right, the results of anti-human CD223, FITC (clone: oti5d8) and Anti-Human CD4, APC are shown. All cells are gated.