Fig.-The results of anti-human ZAP-70 and FITC (clone: 1E7.2) staining on human peripheral blood after anti-human CD3 and PE staining. The lymphocyte in FSC/SSC was gated for analysis.

Overview of ZAP70 molecular target information

Molecular name: zap70, zeta chain of t cell receiver associated protein kinase 70.

Gene family: SH2 domain containing; Syk family tyrosine kinases

Alias: ZAP-70; STD

Used name: SRK

Full name: tyrosine-protein kinase ZAP-70; SRK

Review of ZAP70 molecular targets

TCR-related protein -70, expressed in T cells and NK cells, is necessary for TCR signal transmission and development. ZAP-70 interacts with TCR complex by binding to ITAMs existing in the invariant subunit of TCR complex. After activation, ZAP-70 is phosphorylated on several tyrosine residues through two mechanisms: autophosphorylation and trans phosphorylation of Src family tyrosine kinase Lck1-3. Tyrosine phosphorylation of ZAP-70 is related to its increased kinase activity and triggers downstream signal events. Although ZAP-70 is usually expressed in T cells and NK cells, several recent studies also show that the positive expression of ZAP-70 is highly correlated with the expression of mutant IgVH in B chronic lymphoblastic leukemia (CCL). In a word, the expression of ZAP-70 can be measured by intracellular flow cytometry, which can be used as a prognostic indicator of B-CLL.