Principle: Adopting biphasic fixation system of ethanol-formalin (liquid A) and acetic acid (liquid B), ethanol rapidly penetrates and dehydrates, formalin effectively cross-links the proteins, and acetic acid regulates the pH and enhances the softening of the tissues, the synergistic effect of the three prevents contraction and deformation of the worm body, which is especially suitable for fixation of parasites with thicker body walls.

Main components: Liquid A: ethanol, formalin; Liquid B: acetic acid.