Bacterial flagellum is the organ of bacterial movement, Helicobacter pylori can pass through the mucus layer on the gastric epithelial cells from the strongly acidic gastric lumen to reach the neutral environment of the gastric epithelial cells, which is a good example of the role of flagellar movement, through flagellar staining you can observe the flagellar morphology, the number of flagellum and flagellum in the distribution of the location of the bacteria, the flagellum number and distribution of the location of the flagellum in the bacteria is one of the important bases of identification of the bacteria.

The flagellar staining solution was adopted the modified Ryu staining method, the advantages of this staining method are the use of crystal violet as the core dye, the reagent is more stable, the operation is simple, and the results are more reliable.