Brucella (Brucella) is a gram-negative non-motile bacteria, cattle, sheep, pigs and other animals are most susceptible to infection, Brucella staining is also known as Kozlowski stain or Kozlowski stain.

Brucella staining solution adopts sand yellow as the stain, clinical specimens are directly smeared, the background is clean, the nucleus and cytoplasm are in strong contrast, the intracellular phagocytosis is clear and easy to recognize, and the bacterial staining characteristics are typical. All operations should be placed in a secondary biological safety cabinet, once the green bacteria are found to have red dots of bacteria, the bacteria should be highly suspected to be Brucella, and CDC should be notified in time to prevent the spread of the epidemic.