Viral hepatitis is an infectious disease caused by hepatitis viruses with degeneration and necrosis of liver parenchymal cells as the main lesion. Hepatitis B virus is the hepatitis B virus that spreads worldwide, HBsAg exists in the cytoplasm of the liver cells, nowadays, HBsAg and HBcAg of hepatitis B virus can be shown by immunohistochemistry, the methods to show the HBsAg are Shikata lichen reds, aldehyde magenta method, Victoria blue method and so on.

The virus staining solution (aldehyde magenta method) is easy to operate and has a short staining time, and the possible mechanism is that mature aldehyde magenta has a strong affinity for specific proteins and sulfate-containing mucopolysaccharides, and binds well to HBsAg.