Heterochromatic particle staining is now often used Albert method, which is simple, clear contrast, and mostly used for diphtheria rod-shaped bacilli staining. Corynebacterium diphtheriae is mostly rod-shaped, gram-positive bacteria, but often show one end of the irregular extension into a rod, the bacteria are often gathered in clusters at an acute angle into a V, L or Y type, no motility, pods, bud cells, staining of the bacterial body at one end, the two ends or the center of the obvious deep dye particles, that is, the heterogeneous dyeing particles.

The staining solution is a modified Albert's method, after staining of Corynebacterium diphtheriae, obvious deep stained particles, i.e. heterochromatic particles, are visible at one end, both ends or the center of the bacterium.