Pathogenic nematodes in diseased tissues can be obtained from live nematodes by direct picking with a needle, funnel separation or mashing the diseased tissues for separation, etc. In order to identify common nematodes and to make general observations on their morphology and structure, temporary slides can be made from live nematodes, and in order to further scrutinize and recognize their taxonomic characteristics, live nematodes need to be killed and fixed, and sometimes stained, to be made into permanent slide specimens, and in order to To observe the interrelationship between nematode and host tissues, paraffin sections of host and nematode tissues are also made.

The staining solution is mainly composed of hematoxylin and lichen red, etc. It is mainly used for observing the staining of free living nematodes, which is first stained with hematoxylin and then restained with lichen red.