The neuronal cell body consists of a large nucleus with a ruffled nuclear membrane, sparse chromatin, and a conspicuous nucleolus, in which the cytoplasm is composed of Nysted granules, i.e., basophilic granules that are representative of the rough endoplasmic reticulum and that give rise to specific patchy basophilic manifestations in many neurons. Nissl granules can be visualized with many stains such as neutral red, methylene blue, toluidine blue, and methyl violet, and the variability of staining, pH, and time of differentiation allows some stains to highlight both the Nissl material alone and the nucleus and glia of the neuron; Nissl bodies, or Nissl vesicles, are small triangular or oval masses distributed in the cytoplasm of the neuronal cell that are able to be Nissl bodies, or nissl bodies, are triangular or oval-shaped masses distributed in the cytoplasm of nerve cells and can be stained a purplish-blue color by basic dyes such as sulfur cordial, methylene blue, toluidine blue, and tar violet, etc. Nissl bodies are found in all kinds of nerve cells, but their shapes, numbers, and distribution locations are often different. Nystrophinosomes change due to changes in physiological status. Nystrophinosomes are an important site for synthesizing protein synthesis within the neuron, and when the neuron is stimulated, the number of nystrophinosomes within the envelope decreases markedly.

Tar violet staining solution (0.5%) with imported tar violet as the core dye, tar violet has a light-sensitive effect, can well show the changes in the nidus, this reagent is easy to operate, stable staining, wide range of application, can be used for staining of nidus substances, neurons, etc. in paraffin tissue sections; the presence and disappearance of nidus is an important indicator of whether the nerve cells are damaged, when encephalitis, cerebral ischemia, axon reaction When encephalitis, cerebral ischemia, axonal reaction, etc. occurs, the nidus will dissolve or even disappear.