Hematoxylin (Hematoxylin) and Eosin (Eosin) co-staining referred to as HE staining is one of the most commonly used staining methods in pathology and histology. Hematoxylin is an alkaline natural dye that can color the cell nucleus. The main component of chromatin in the cell nucleus is DNA, and in the double helix structure of DNA, the phosphate groups on the two nucleotide chains are outwardly oriented, which makes the outside of the DNA double helix negatively charged and acidic, and it can easily be stained by combining with the positively charged Hematoxylin alkaline dye by ionic or hydrogen bonding.

Carazzi hematoxylin staining solution is mainly composed of hematoxylin, potassium aluminum sulfate, etc., belonging to a kind of alum hematoxylin, the staining solution has a small content of hematoxylin, no oxidized membrane formation, the staining of the cell nucleus is very clear, without staining the cytoplasm and the fiber components, it is a progressive staining, so the staining does not need hydrochloric acid ethanol differentiation, the staining time is about 8 to 10 min, the staining solution is similar to Mayer hematoxylin staining solution, the staining solution is similar to Mayer hematoxylin staining solution. This staining solution is similar to Mayer Hematoxylin Staining Solution, which can be used for special staining of glycogen, staining of enzyme histochemistry and immunohistochemistry and other staining of the cell nucleus, especially suitable for staining of the cell nucleus after special staining can not be processed by the acid treatment, at this time, the time used is shorter (usually 8~10min), and can be bluecolored after staining, no need to be differentiated.