Adenosine triphosphatase (ATPase) is a hydrolytic enzyme that catalyzes the synthesis of ATP. ATPase is classified into membrane adenosine triphosphatase, myosin adenosine triphosphatase, mitochondrial adenosine triphosphatase and so on according to the activator, inhibitor and enzyme localization, ATPase can hydrolyze ATP to adenosine phosphate and phosphate. Adenosine triphosphate is hydrolyzed to adenosine diphosphate and phosphoric acid, this enzyme only acts on the high energy bond between phosphoric acid and phosphoric acid, thus releasing a large amount of energy, and its catalytic reaction is as follows: A-P-P-P + H2O → A-P-P + H3PO4 + energy

ATPase staining solution (calcium-cobalt method) The principle of the ATPase is that the adenosine triphosphate enzyme hydrolyzes adenosine triphosphate to adenosine diphosphate and phosphoric acid, and the root of phosphoric acid and calcium ions are combined to calcium phosphate precipitation, and then sequentially The phosphate is combined with calcium ions to form a calcium phosphate precipitate, which is then sequentially replaced by cobalt phosphate and cobalt sulfide, with the final product being a black cobalt sulfide precipitate.