Glucose-6 -phosphatase (G-6-Pase) is a membrane-bound enzyme mostly found in mammalian tissues such as liver, kidney, and intestine, etc. G-6-Pase is tightly bound to microsomes, localized in the endoplasmic reticulum, and is the main marker enzyme of the endoplasmic reticulum, which is able to hydrolyze glucose-6-phosphate into glucose and phosphoric acid.G-6-Pase has a crucial role in the G-6-Pase is a key enzyme in glucose metabolism and plays a crucial role in maintaining the relative constancy of blood glucose concentration. When blood glucose decreases, G-6-Pase promotes the conversion of hepatic glycogen into blood glucose, and the lack of G-6-Pase will cause impaired glycogen decomposition, causing accumulation of glycogen in the liver, kidneys, heart and other parts of the body and leading to hepatic glycogenosis; the optimal pH of G-6-Pase is 6.5, and it can also be hydrolyzed in the range of pH 6.0-8.0, and pH 8.0 can also be used as an inhibitor of glucose. The most suitable pH for G-6-Pase is 6.5, and it can also be used at pH6.0～8.0. pH8.0 is the most stable, and pH5.0 is easy to be denatured, while pH6.5～6.7 is used for histochemical reaction.

Glucose-6-Phosphatase Staining Solution (Lead Method) adopts the combination of heavy metal trapper and phosphoric acid to show the enzyme's activity, and the enzyme is very sensitive to fixation, and the enzyme will be completely inhibited by paraffin embedding after fixation of tissues in 80% acetic acid, which will be used for fresh tissues in low-temperature and constant-cold slices. Formaldehyde short time fixation enzyme is inactivated, but can be short time low temperature acetone fixation, but generally not fixed.