Muscle fiber (Muscle fiber) belongs to the muscle tissue components, composed of myocytes, according to the morphological and functional characteristics, muscle fiber can be divided into smooth muscle (also known as transverse muscle), skeletal muscle, cardiac muscle. Myofiber staining methods are many kinds of methods, such as Li Chun red method, aniline blue method, tungsten phosphotungstic acid hematoxylin method, etc., the initial invention of phosphotungstic acid hematoxylin staining solution, Mallory's PTAH method there are a variety of tungsten phosphoric acid hematoxylin method, in 1900 or so Mallory will be phosphotungstic acid aqueous solution and hematoxylin staining solution associated with the discovery of the method for myofibers staining is better, and is now widely used is the Mallory phosphotungstic acid hematoxylin staining solution (PTAH natural oxidation method). Hematoxylin can be chemically oxidized by PTAH, but the effective period is shorter and the staining power is easy to decline, although the natural oxidation is time-consuming, but the hematoxylin can be used for more than 2 years, and the staining power is not easy to be lost, it is a more ideal staining solution for CNS, general tissue structure and all the standard fixative fixed tissues, the staining time depends on the method of preparation, the use of the fixative and the structure of the tissue shown.

Mallory PTAH staining solution (natural oxidation method), also known as Mallory Hematoxylin Phosphotungstate Staining Solution, is a naturally mature staining solution, with better staining power and long preservation time, which is mostly used to show the transverse striations of rhabdomyosarcoma, and clinically it is applied to the diagnosis of rhabdomyosarcoma, which is difficult to distinguish from undifferentiated mesenchymal tumors because of its varied histological morphology; after staining, if the tumor cells are found to be in a different state, it will be difficult to identify them with hemangioma; Hematoxylin Phosphotungstate Staining Solution will be used. After staining, if blue transverse stripes are found in the cytoplasm of tumor cells, it can be proved that the tumor is rhabdomyosarcoma differentiated; this staining solution can also stain the fibrin of inflammatory exudate, fibrin in the capillaries of DIC, and neuropathological aspects.