Muscle fiber (Muscle fiber) belongs to the muscle tissue components, composed of myocytes, according to the morphology and functional characteristics, muscle fibers can be divided into smooth muscle (also known as transverse muscle), skeletal muscle, cardiac muscle, muscle fiber staining methods have a variety of methods, such as Li Chun red method, aniline blue method, tungsten phosphotungstic acid hematoxylin method, and so on. Initially invented phosphotungstic acid hematoxylin staining solution, Mallory's PTAH method there are a variety of tungsten phosphoric acid hematoxylin method, around 1900 Mallory will be phosphotungstic acid aqueous solution and hematoxylin staining solution, found that the method is better for myofibrillar staining, and is now widely used that is the Mallory Phosphotungstic acid hematoxylin staining solution (PTAH natural oxidation); hematoxylin available Hematoxylin can be chemically oxidized by PTAH, but the effective period is shorter and the staining power is easy to decrease. Although natural oxidation is time-consuming, the hematoxylin produced can be used for more than 2 years, and the staining power is not easy to lose, it is a more ideal staining solution for CNS, general tissue structure, and all standard fixative-fixed tissues, and the staining time varies according to the method of preparation, the fixative used, and the tissue structure shown.

Mallory PTAH staining solution (chemical oxidation method), also known as Mallory phosphotungstic acid hematoxylin staining solution, is a chemical oxidant catalyzed staining solution, the staining power is better in a short period of time, and the preservation time should not be too long, and it is mostly used to show the transverse striations of the transverse muscle, which is clinically applied to the diagnosis of rhabdomyosarcoma. The histological morphology of rhabdomyosarcoma is varied, and it is difficult to distinguish it from undifferentiated mesenchymal tumors; after staining with phosphotungstic acid hematoxylin, if blue transverse stripes are found in the cytoplasm of tumor cells, it can be proved that the tumor is showing rhabdomyosarcoma differentiation, and this staining solution can also be stained for the fibrils in the exudate from the inflammation, the fibrils in the capillaries in the DIC, as well as in neuropathology, and so on.