Muscle fiber (Muscle fiber) belongs to the muscle tissue components, composed of myocytes, according to the morphology and functional characteristics, muscle fibers can be divided into smooth muscle (also known as transverse muscle), skeletal muscle, cardiac muscle, muscle fiber staining methods have a variety of methods, such as Li Chun red method, aniline blue method, tungsten phosphotungstic acid hematoxylin method, etc.; the initial invention of the phosphotungstic acid hematoxylin staining solution, Mallory's PTAH method There are a variety of tungsten phosphoric acid hematoxylin method, around 1900 Mallory will be phosphotungstic acid aqueous solution and hematoxylin dye solution, found that the method for muscle fiber staining is better, and is now widely used that is Mallory Phosphotungstic Acid Hematoxylin Dyeing Solution (PTAH natural oxidation); Hematoxylin can be used in PTAH chemical oxidation, but the validity of the short period of time and easy to decline in the staining power. Although natural oxidation is time-consuming, the hematoxylin produced can be used for more than 2 years, and the staining power is not easy to lose, it is a more ideal staining solution for CNS, general tissue structure and all standard fixative-fixed tissues, the staining time varies according to the method of preparation, the use of fixative and the structure of the tissue shown.

Mallory phosphotungstic acid hematoxylin staining solution (PTAH chemical oxidation method) mainly consists of PTAH oxidizing agent, oxalic acid solution, Mallory PTAH staining solution, Mallory PTAH staining solution is a chemically catalyzed staining solution, the staining power is better in a short period of time, and the preservation time should not be too long, it is mostly used for displaying the transverse striations of the transverse myotomes, and it is used to make a diagnosis of rhabdomyosarcoma with the method. The histological morphology of rhabdomyosarcoma is varied, and it is difficult to distinguish it from undifferentiated mesenchymal tumors. After staining with phosphotungstic acid hematoxylin, if blue transverse stripes are found in the cytoplasm of the tumor cells, it can be proved that the tumor is showing rhabdomyosarcoma differentiation, and this reagent can also be stained for the fibrils of the exudate from the inflammation, the fibrils in the capillaries of the DIC, and the neuropathological aspects.