Muscle fiber (Muscle fiber) belongs to the muscle tissue components, composed of myocytes, according to the morphological and functional characteristics, muscle fibers can be divided into smooth muscle (also known as transverse muscle), skeletal muscle, cardiac muscle, muscle fiber staining methods have a variety of methods, such as Lichtenstein red, aniline blue, tungsten phosphomolybdic acid hematoxylin method and so on. There are fewer hematoxylins that use phosphomolybdic acid as a mordant, and the only one that has received much recognition is the Thomas phosphomolybdic acid hematoxylin technique used by McManus and Nowry for displaying collagen and coarse reticular fibers.

Thomas phosphomolybdate hematoxylin solution mainly consists of Thomas phosphomolybdate hematoxylin and Thomas differentiation solution, which is mainly used for staining of collagen, coarse reticular fibers, and silver-loving cellular granules.