Muscle tissue biopsy and corresponding special staining can show the structure, type and chemical composition changes of muscle fibers and help to diagnose various types of muscle fiber diseases. Muscle tissue biopsy technology can not only provide a clearer and deeper understanding of the discovered myopathies, but also discover some new myopathies, so that the diagnosis of skeletal muscle diseases can be improved to a new level of combination of morphology and function. Skeletal muscle tissue biopsy should be stained and observed by the method of low-temperature rapid frozen section, in order to truly respond to the various morphological and structural changes of the muscle fibers, to avoid artificial artifacts and loss of enzyme activity due to the process of fixation, dehydration, embedding and heat treatment, etc., and to make the muscle tissues preserve the original structure without being damaged.

Gomori trichrome staining is a multicolor staining method of connective tissue improved on the basis of Masson trichrome staining method, which can make a variety of components of connective tissue coloring, myofibrils are lime green, collagen fibers are bright green, nuclei of cells are purple, mitochondria are red, and the background is green, which makes coloring clear and easy to observe.