Collagen Fiber (Collagen Fiber) is the most widely distributed in the connective tissue with the highest content of a fiber, widely distributed in a variety of organs, of which the skin, sclera, tendons are the most abundant. Type I collagen fibers are mainly bone, skin, tendon fibers; Type II collagen fibers are mainly cartilage collagen; Type III collagen fibers are mainly in embryonic tissues, adult blood vessels, gastrointestinal tract; Type IV collagen fibers are mainly in basement membranes; methods to show collagen fibers include: Van Gieson staining, Masson trichrome staining, Victoria blue collagen fiber staining, Sirius red staining, etc., polarized light microscopy. Collagen fibers have the property of positive uniaxial birefringence of light. Combined with Sirius Red Complex Staining Solution, it enhances birefringence and improves resolution, thus distinguishing between the two types of collagen fibers.

Victoria blue collagen fiber staining solution is mainly composed of Victoria blue staining solution and Lichun red staining solution, which overcomes the shortcomings of VG staining, which is easy to fade and the contrast is not obvious, and it is mainly used in the study of collagen fiber abnormality or fibroplasia in various tissue lesions, and the collagen fibers of the cardiac vasculature and other tissues are stained in red under ordinary optical microscope.