There are various methods of bone staining, such as toluidine blue method, Alisin blue method, saffron O method, Goldner trichrome staining method, etc. And Goldner trichrome staining is also known as Goldner trichrome staining, which is similar to Masson trichrome staining, and the trichrome staining usually refers to staining the nucleus of the cell and the ability to selectively display the collagen fibers and myofibrils.

Goldner trichrome staining solution staining principle is related to the size of the anionic dye molecule and tissue penetration: the size of the molecule is reflected by the molecular weight, small molecular weight is easy to penetrate the structure of dense, low permeability of the tissue; while the large molecular weight can only enter the structure of loose, high permeability of the tissue, the stain is mostly used for the staining of bone substances, the staining of the cell is more effective, especially for metabolic diseases ( Paget's disease, osteodystrophy, etc.) to assess the activity of osteoblasts and osteoclasts and to easily identify metastatic tumor cells in the bone marrow.