Bone tissue is divided into hard bone and cartilage, cartilage tissue consists of chondrocytes and cartilage matrix, cartilage tissue and its surrounding cartilage membrane constitutes cartilage, there are many kinds of bone staining methods, for example, Toluidine blue method, Alisin blue method, Sanguine O method, solid green - Sanguine O staining method, Goldner trichrome staining method, etc., Methylene blue-acidic magenta staining solution can clearly show osteoblasts, cell matrix and osteoid. Methylene blue-acidic magenta stain can clearly show osteoblasts, cellular matrix and osteoid, and is ideal for the study of static parameters of bone formation, such as osteoblast index, osteoid uniform width, osteoid surface and osteoid volume.

After staining with methyl blue-acidic magenta staining solution, the hydroxyapatite coating on the implant surface was purplish-red, the osteoblasts, cell nuclei and osteoid were clearly shown, the nuclei of the osteoblasts were stained dark blue, the cellular matrix was stained light blue, and the osteoid was purplish-gray; the neoosteogenic bone tissue was dark red with clear demarcation of the implant-bone interface, and the protomineralized bone tissue was red with a blurring of the boundary between it and the neoosteogenic bone.