Hoechst 33258 is also known as bisBenzimide H 33258 or HOE 33258, molecular formula C25H24N6O3HCl, molecular weight 533.88, CAS Number 23491-45-4.Hoechst 33258 is a blue fluorescent dye that can penetrate cell membranes, with low toxicity to the cells. Hoechst 33258 is a blue fluorescent dye that can penetrate the cell membrane, with low toxicity to cells, and is commonly used for apoptosis detection, fluorescence microscopy or flow cytometry after staining, and is also used for general nuclear staining and DNA staining.Hoechst 33258 has a maximum excitation wavelength of 346 nm and a maximum emission wavelength of 460 nm. When Hoechst33258 is combined with double-stranded DNA, the maximum excitation wavelength is 352nm and the maximum emission wavelength is 461nm.

Hoechst33258 Staining Solution can be used directly for nucleus staining of fixed cells or tissues, and can also be used directly for nucleus staining of live cells or tissues.