Diff-Quik staining is a rapid staining method improved on the basis of Wright's staining, which is one of the commonly used staining methods in cytological examination. The staining solution is prepared by the rapid staining method recommended by the World Health Organization (WHO), and similar to Wright's Stain, it is improved by using the principle of Romanowsky Stain technology, and the results are extremely similar to Rachel's Stain. The staining results are very similar to those of Rachel's stain, but the time required for Dove's rapid staining is very short, and the staining can be completed in less than 90 s. The sperm morphology can be assessed by Dove's rapid staining.

Sperm morphology can be evaluated by Diff-Quik stain. Sperm morphology quick stain (Diff-Quik method) belongs to the sperm morphology stain recommended by WHO, and its staining principle is that proteins with different isoelectric points in spermatozoa and cells have different charges under the same acidity, and they can selectively bind to the corresponding dyes and be colored; the amino group of eosinophilic proteins dissociates with a positive charge, which can bind to the negatively charged acidic dye and be colored; the amines of eosinophilic proteins dissociate with positive charges, which can bind to the negatively charged acidic dye. acidophilic proteins dissociated amino group is positively charged, can be combined with negatively charged acidic dyes (e.g. acidophilic xanthene, eosinophilic red) and colored red; basophilic proteins dissociated carboxyl group is negatively charged, can be combined with positively charged basic dyes (basophilic thiazolidine, methylene blue) and colored blue; neutrophilic proteins dissociated amino group and negatively charged carboxyl group are equal, and at the same time, combined with the equal number of acidic dyes and basic dyes to show violet-red color, but Because of the equal dissociation charge, the coloring is weak, mainly used for spermatozoa (cell) morphology assessment, containing fixative, very suitable for batch immersion dyeing, and the background is clear and unsinkable.