Ricci pigment is a composite dye composed of acidic dye Eosin and basic dye Methylene Blue, which has a good differentiation effect on the staining of protoplasm. Various cell components with different chemical properties, the affinity for various dyes is not the same, such as hemoglobin, eosinophilic particles for basic proteins, and acidic dye eosin combination, dyeing pink, known as eosinophilic substances; nucleolar proteins, lymphocytes, basophilic granulocytes cytoplasm is acidic, and the combination of basic dyes, dyeing violet blue or blue, known as basophilic substances; neutral particles are isoelectric state and eosin and Methylene Blue can be combined, dyeing light. Neutrophilic granules are isoelectric and bind to both eosin and methylene blue, staining a light purplish-red color, and are called neutrophils. Primitive erythrocytes, early juvenile erythrocytes cytoplasm, nucleus contains more acidic substances, stained into a thicker blue; middle juvenile erythrocytes contain both acidic and alkaline substances, stained red-blue or grayish-red; fully mature erythrocytes, after the complete disappearance of the acidic substances, stained pink. xx staining solution is a blue, clear liquid, with a specific aroma of methanol, mainly consisting of Meiban, eosin, methanol, and so on, wherein methanol's role is to It is mainly composed of Meilan, Eosin and methanol, in which the role of methanol is to dissolve Meilan and Eosin as well as fix the cell morphology.

Characteristics of the dye solution: it is composed of high concentration of Riesling pigment and color lining agent, does not contain phosphate buffer, and can be used directly; trace neutral glycerol is added to the dye solution to prevent volatilization or oxidation of methanol, and it can also make the blood cell staining clearer, and it is often used for staining of blood and cell smears, bone marrow cell smears, and bacterial staining; the cytoplasm of the cells is red, the nucleus of the cells and the bacteria are blue, and the eosinophilic particles are orange-red.