Clone number: RM193

Isotype: rabbit monoclonal antibody

Cellular localization: nucleus

Adaptive tissue: paraffin/frozen

Positive control: Chondroblastoma

Antibody incubation time: 30-60min

Antigen repair: Heat repair (EDTA)

Chondroblastoma is composed of densely arranged chondroblasts, varying numbers of osteoclast-like giant cells, and calcified cartilage-like matrix. H3F3A and H3F3B are two genes encoding variants of histone H3.3. In 95% of chondroblastoma cases, a lysine 36 to methionine (K36M) mutation is present in either H3F3A or H3F3B, with the mutation primarily located in H3F3B. Studies have shown that immunohistochemical detection of the H3.3K36M mutant protein exhibits high specificity and sensitivity in chondroblastoma, with a sensitivity of 95.6% and specificity of 100%, making it highly valuable in clinical practice.

The H3K36M antibody reagent specifically binds to the H3K36M molecular antigen. The immunohistochemistry kit containing the H3K36M antibody reagent is suitable for the precise diagnosis of chondroblastoma.