Principle: Regaud's fixative achieves its fixation effect through the synergistic action of solution A (potassium dichromate solution) and solution B (formaldehyde). Potassium dichromate acts as an oxidizing agent that binds to the amino and sulfhydryl groups of proteins while protecting the lipid structure, while formaldehyde stabilizes the cytoskeleton by cross-linking protein molecules. This dual mechanism of action is particularly favorable to the structural preservation of membrane organelles.

Main components: Liquid A: potassium dichromate solution; Liquid B: formaldehyde