Clone Number: EPNCIR111A

isotype: rabbit monoclonal antibody

Cellular localization: nucleus

tissue: paraffin/frozen

Positive control: appendix

Antibody incubation time: 30-60min

Antigen repair: Heat repair (EDTA)

BRG1/SMARCA4 is a member of the ATP-dependent chromatin remodeling complex family and plays a crucial role in a series of important processes such as proliferation, differentiation, and DNA damage. Members of the chromatin remodeling complex family are composed of three types of subunits: ATPase subunits BRG1 (SMARCA4)/BRM (SMARCA2); highly conserved core subunits INI1 (SMARCB1, SNF5, and BAF47), BAF155 (SMARCC1), and BAF170 (SMARCC2); and functionally specific auxiliary subunits such as PBRM1 (BAF180) and ARID1A (BAF250A). BRG1 is expressed in many normal epithelial tissues. In ovarian tumors, it is absent in most high-calcium-type small cell carcinomas, some ovarian clear cell carcinomas, and uterine leiomyosarcomas, as well as in some adrenal carcinomas, lung carcinomas, medulloblastomas, and pancreatic carcinomas. Approximately 50% of undifferentiated carcinomas show loss of BRG1 expression. The loss of BRG1 expression is associated with reduced survival rates in cancer patients and has prognostic significance.

The SMARCA4/Brg1 antibody reagent specifically binds to the SMARCA4/Brg1 molecular antigen. The immunohistochemistry kit containing the SMARCA4/Brg1 antibody reagent is suitable for the precise diagnosis of SMARCA4-deficient tumors (such as ovarian hypercalcemia-type small cell carcinoma and thoracic SMARCA4-deficient undifferentiated tumors).