Clone number:

Isotype:

Cell location: Cell nucleus

Suitable tissue: Paraffin/frozen

Positive control: Tonsil

Antibody incubation time: 30-60 min

Antigen retrieval: Heat retrieval (EDTA)

T-bet transcription factor is expressed in CD4-positive T lymphocytes and plays a crucial role in the differentiation of immature T helper precursor cells (Thp) into T helper 1 (Th1) cells and the re-differentiation of Th2 cells into Th1 cells. T-bet serves as a reference marker for mature T cells, with minimal expression in Thp and no expression in precursor T-cell leukemia. In reactive lymphoid tissues such as tonsils, lymph nodes, and spleen, T-bet is positively expressed in dispersed small lymphocytes in the follicular zone of T-cell areas, while no expression is observed in the germinal center, perifollicular zone, or marginal zone. T-bet is expressed in B-cell lymphoproliferative disorders, particularly during early B-cell development, including precursor B-cell lymphoblastic leukemia, mature B-cell lymphoma (including chronic lymphocytic leukemia and small lymphocytic leukemia), marginal zone lymphoma, and hairy cell leukemia. Conversely, B-cell lymphomas originating from the pre-germinal center and germinal center are T-bet-negative, including mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma. Therefore, T-bet can serve as a reference for classifying B-cell and T-cell proliferative disorders.

T-bet antibody reagents can specifically bind to T-bet molecular antigens. Immunohistochemistry kits containing T-bet antibody reagents are suitable for the auxiliary diagnosis of T-cell lymphoma.