In well-differentiated liposarcomas with a mucinous matrix, the DDIT3 gene may exhibit amplification but not translocation, necessitating differentiation from mucinous liposarcomas. When using the DDIT3 breakage probe for initial testing, if the result is translocation-negative but shows amplification at the probe end toward MDM2 (red in our company's probe) or throughout the entire red-green probe, it is highly suspected that the DDIT3 amplification is caused by MDM2 amplification, as both are located in the 12q13-15 region and are closely spaced (only 12M apart). Additionally, this probe can distinguish DDIT3 amplification from CDK4 amplification.