Hematoxylin (Hematoxylin) and Eosin (Eosin) co-staining, referred to as HE staining, is one of the most commonly used stains in pathology and histology. Hematoxylin is a basic natural dye that stains the nucleus of cells. The main component of chromatin in the cell nucleus is DNA. In the double helix structure of DNA, the phosphate groups on the two nucleotide chains are outwardly oriented, which makes the outside of the DNA double helix negatively charged and acidic, and it can be easily stained by combining with the positively charged hematoxylin alkaline dyes by ionic bonding or hydrogen bonding.

Mayer hematoxylin staining solution belongs to a kind of alum hematoxylin, hematoxylin content is small, no oxidized membrane formation, the cell nucleus staining is very clear, not staining cytoplasmic and fibrous components, is a progressive staining, so the staining does not need to be hydrochloric acid ethanol differentiation, the staining time is about 3 to 5 min. this reagent is commonly used in the special staining such as glycogen, enzyme histochemistry and immunohistochemistry staining after the re-staining of the cell nucleus, is particularly suitable for the staining of cell nucleus in the case of after special The reagent is often used for the re-staining of cell nucleus after special staining such as glycogen, enzyme histochemistry and immunohistochemistry, especially suitable for the re-staining of cell nucleus after special staining when it can not be processed by acid treatment, at this time the staining time is shorter (usually 5~10min), and it can be bluechemistry after staining without differentiation.